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Intervention and Therapy for Pleural Effusions


Wednesday, November 2, 2005

12:30 PM - 2:00 PM

A NEW, RELIABLE MODEL OF EXPERIMENTAL PLEURODESIS IN MICE

Evaldo Marchi, MD*, Francisco S. Vargas, MD, Milena M. Acencio, BS, Gabriela G. Carnevale, BS, Leila Antonangelo, MD, Eduardo H. Genofre, MD and Lisete R. Teixeira, MD

Pulmonary Division, InCor and University of Sao Paulo Medical School, Sao Paulo, Brazil

PURPOSE: The experimental model of pleurodesis in rabbits has been useful in understanding the pathophysiology of the pleural inflammatory injury induced by several sclerosing agents. However, restrictions for the use of rabbits in laboratory investigation are making this model less accessible. The aim of this study is to present a new experimental model of pleurodesis in mice using talc or silver nitrate.

METHODS: Two groups of ten C57BL/6 mice received 0.5mL intrapleurally of talc 4mg/gr or 0.05% silver nitrate. After 28 days the animals were sacrificed and the pleural cavity was opened and evaluated for evidence of macroscopic pleural adhesions and hemothorax (scores 0 to 4), atelectasis (scores 0-2) and microscopic pleural and alveolar inflammation and fibrosis (scores 0 to 4). Statistics: student t-test.

RESULTS: Although both agents produced an efficient pleurodesis, silver nitrate was more effective than talc, with significant higher scores for pleural adhesions, microscopic pleural inflammation and fibrosis. Hemothorax, atelectasis and microscopic alveolar inflammation and fibrosis were negligible in both groups.

CONCLUSION: Either talc 4mg/gr or 0.05% silver nitrate produced an efficient pleurodesis in our experimental model in mice.



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DISCLOSURE: Evaldo Marchi, None.

CLINICAL IMPLICATIONS: We describe a new model of pleurodesis that may overcome the restrictions to the use of large and medium-sized animals in laboratory investigation.






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